PCR Primers for Alternative Splicing

SpliceExpress™ PCR is the first collection of PCR primers designed to detect alternative splicing for the whole human genome.

A single microarray experiment can identify thousands of probes differentially expressed between disease and normal samples. Quantitative PCR is the method of choice to validate biomarkers for use as diagnostics or drug targets. But designing PCR primers can be laborious, especially when the biological complexity of RNA splicing is taken into account, slowing the translation of genomic information into clinic. Jivan's genome-wide PCR primer set simplifies and expedites this process.

Customers for Jivan's SpliceExpress™ splice arrays can order the corresponding PCR primer pair for any oligonucleotide probe on any SpliceExpress™ array. Alternatively, researchers can directly order PCR primers for genes and splice variants of interest, bypassing microarray screening entirely.

606,418 PCR primer pairs for human exon junctions and exons
Available per primer pair, per gene or per array
195,127 alternatively spliced sites and regions
98,382 splice isoforms
23,484 human genes from the Human Genome Project, RefSeq and EST libraries
Primer length: 24 bases (average 24, min 21, max 27)
GC content: 52% (average)
T_m: 60°C (average)
PCR product length: 231 bases (average)
Catalog number: TEPCR

Full service quantitative rt-PCR is also available.

Questions? Contact us.