PCR Primers for Alternative Splicing

Jivan's SplicePrimer™ PCR primers were the first collection of PCR primers designed to detect alternative splicing for the whole human genome, launched in 2005. The current release detects more splice isoforms than ever before, and with greater sensitivity.

Product Details

• 606,418 PCR primer pairs for human exon junctions and exons
• Available per primer pair, per gene or per array
• 195,127 alternatively spliced sites and regions
• 98,382 splice isoforms
• 23,484 human genes from the Human Genome Project, RefSeq and EST libraries
• Primer length: 24 bases (average 24, min 21, max 27)
• GC content: 52% (average)
• T_m: 60°C (average)

A single microarray experiment can identify thousands of probes differentially expressed between disease and normal samples. PCR is the method of choice to validate biomarkers for use as diagnostics or drug targets. But designing PCR primers for quantitative PCR (qPCR) or conventional PCR can be laborious, especially when the biological complexity of RNA splicing is taken into account, slowing the translation of genomic information into clinic. Jivan's genome-wide PCR primer set simplifies and expedites this process.

Customers for Jivan's SpliceExpress™ splice arrays can order the corresponding PCR primer pair for any oligonucleotide probe on any SpliceExpress™ array. Alternatively, researchers can directly order PCR primers for genes and splice variants of interest, bypassing microarray screening entirely.