Giemsa Solution
Properties
Write: (for research use only)
Quality level: 200
Duration: limited shelf life
Technique(s)
microbe identification | staining: Adequate
Suitability
- Chlamydia spp.
- Histoplasma spp.
- Plasmodium spp.
- Trichomonas spp.
- bacteria
- protozoa
- spirochetes
Chain of smiles
[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1
InChI
1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3 -8,15H,1-2H3;1H
inChI key
NALREUIWICQLPS-UHFFFAOYSA-N
Request
Tested for staining blood smears according to G. Clark (ed.), Staining Procedures, 4th Edition, Williams & Wilkins, 173 (1981); together with May-Grünwald’s solution for Papanicolaou staining according to Pappenheim.
Caution
Store at 20°C
Beginning
When blood smears are stained with Giemsa stain, the nucleus and cytoplasm of the white blood cells take on a characteristic blue or pink colouration. The use of purified eosin and thiazine stains minimizes lot-to-lot variation.
Physical form
Solution of blue B/blue II-eosin/methylene blue 1:12:2 (w/w/w) in glycerol/methanol 5:24 (v/v); total dye content: 0.6% (w/w)
Reconstitution
Fix 10 min. in methanol; air dry; soak 45 min. in Giemsa solution; rinse with distilled water; air dry
