Biologic Pathways Underlying Prognostic Radiomics Phenotypes from Paired MRI and RNA Sequencing in Glioblastoma
- Jivan
- 0
Background The biologic that means of prognostic radiomics phenotypes stays poorly understood, hampered partially by lack of multicenter reproducible proof. Objective To uncover the biologic that means of particular person prognostic radiomics phenotypes in glioblastomas utilizing paired MRI and RNA sequencing information and to validate the reproducibility of the recognized radiogenomics linkages externally.
Supplies and Strategies This retrospective multicenter examine included 4 information units gathered between January 2015 and December 2016. From a radiomics evaluation set, a 13-feature radiomics signature was constructed utilizing preoperative MRI for total survival prediction.
Utilizing a radiogenomics coaching set with each MRI and RNA sequencing, biologic pathways had been enriched and correlated with every of the 13 radiomics phenotypes. Radiomics-correlated key genes had been recognized to derive a prognostic radiomics gene expression (RadGene) rating.
The reproducibility of recognized pathways and genes was validated with an exterior check set and a public information set (The Most cancers Genome Atlas [TCGA]). A log-rank check was carried out to evaluate prognostic significance. Outcomes A complete of 435 sufferers (imply age, 55 years ± 15 [standard deviation]; 263 males) had been enrolled. The radiomics signature was related to total survival (hazard ratio [HR], 3.68; 95% CI: 2.08, 6.52; P < .001) within the radiomics validation subset.
4 kinds of prognostic radiomics phenotypes had been correlated with distinct pathways: immune, proliferative, remedy responsive, and mobile features (false-discovery price < 0.10). Thirty radiomics-correlated genes had been recognized. The prognostic significance of the RadGene rating was confirmed in an exterior check set (HR, 2.02; 95% CI: 1.19, 3.41; P = .01) and a TCGA check set (HR, 1.43; 95% CI: 1.001, 2.04; P = .048).
The radiomics-associated pathways and key genes might be replicated in an exterior check set. Conclusion Particular person radiomics phenotypes on MRI scans predictive of total survival had been pushed by distinct key pathways concerned in immune regulation, tumor proliferation, remedy responses, and mobile features in glioblastoma, which may very well be reproduced externally. © RSNA, 2021 On-line supplemental materials is offered for this text.
Nonoperative and Operative Bone and Cartilage Regeneration and Orthopaedic Biologics of the Hip: An Orthoregeneration Community (ON) Basis Hip Evaluation
Orthoregeneration is outlined as an answer for orthopedic situations that harnesses the advantages of biology to enhance therapeutic, scale back ache, enhance perform, and optimally, present an setting for tissue regeneration. Choices embody: medication, surgical intervention, scaffolds, biologics as a product of cells, and bodily and electro-magnetic stimuli.
The purpose of regenerative medication is to reinforce the therapeutic of tissue after musculoskeletal accidents as each remoted remedy and adjunct to surgical administration, utilizing novel therapies to enhance restoration and outcomes.
Varied orthopaedic biologics (orthobiologics) have been investigated for the remedy of pathology involving the hip, together with osteonecrosis (aseptic necrosis) involving bone marrow, bone, and cartilage, and chondral accidents involving articular cartilage, synovium, and bone marrow.
Promising and established remedy modalities for osteonecrosis embody non-weight bearing; pharmacological remedies together with low molecular-weight heparin, prostacyclin, statins, bisphophonates, and denosumab, a receptor activator of nuclear factor-kB ligand (RANKL) inhibitor; extracorporeal shock wave remedy; pulsed electromagnetic fields; core decompression surgical procedure; mobile therapies together with bone marrow aspirate (BMA) comprising mesenchymal stromal cells (MSCs aka mesenchymal stem cells) and bone marrow autologous focus (BMAC), with or with out expanded or cultured cells, and potential addition of bone morphogenetic protein-2 (BMP-2), vascular endothelial progress issue (VEGF), and primary fibroblast progress issue (bFGF); and arterial perfusion of MSCs which can be mixed with addition of carriers or scaffolds together with autologous MSCs cultured with beta-tricalcium phosphate (b-TCP) ceramics related to a free vascularized fibula.
Promising and established remedy modalities for chondral lesions embody autologous platelet-rich plasma (PRP); hyaluronic acid (HA); MSCs (in expanded or non-expanded kind) derived from bone marrow or different sources resembling fats, placenta, umbilical wire blood, synovial membrane, and cartilage; microfrature or microfracture augmented with membrane containing MSCs, collagen, HA, or artificial polymer; mosaicpasty; one-stage autologous cartilage translation (ACT) or two-stage ACT utilizing three-dimensional spheroids; and autologous cartilage grafting; chondral flap restore, or flap fixation with fibrin glue.
Hip ache is catastrophic in younger sufferers, and promising therapies supply a substitute for untimely arthroplasty. This will handle each bodily and psychological parts of ache the purpose is to keep away from or postpone a man-made joint. LEVEL OF EVIDENCE: Degree V, knowledgeable opinion. Hip Orthoregeneration for Osteonecrosis and Chondral Defects.
Direct concentrating on of TDP-43, from small molecules to biologics: the therapeutic panorama
Tar DNA binding (TDP)-43 proteinopathy, sometimes described as cytoplasmic accumulation of extremely modified and misfolded TDP-43 molecules, is attribute of a number of neurodegenerative illnesses resembling Amyotrophic Lateral Sclerosis (ALS) and limbic-predominant age-related TDP-43 encephalopathy (LATE). TDP-43 proposed proteinopathies embody homeostatic imbalance between nuclear and cytoplasmic localization, aggregation of ubiquitinated and hyper-phosphorylated TDP-43, and a rise in protein truncation of cytoplasmic TDP-43.
Given the therapeutic curiosity of concentrating on TDP-43, this evaluation focuses on the present panorama of methods, starting from biologics to small molecules, that straight goal TDP-43. Antibodies, peptides and compounds have been designed or discovered to acknowledge particular TDP-43 sequences however alleviate TDP-43 toxicity by means of totally different mechanisms.
Whereas two antibodies described right here had been in a position to induce degradation of pathological TDP-43, the peptides and small molecules had been primarily designed to scale back aggregation of TDP-43. Moreover, we talk about promising rising therapeutic targets.
Frailty, ageing, and periodontal illness: Primary biologic concerns
Getting old is related to the event of illness. Periodontal illness is without doubt one of the many illnesses and situations that enhance in prevalence with age. Along with the normal give attention to particular person age-related situations, there may be now a higher recognition that multisystem situations resembling frailty play an vital position within the well being of older populations.
Frailty is a scientific situation in older adults that will increase the danger of opposed well being outcomes. Each frailty and periodontal illness are widespread power situations in older populations and share a number of danger elements.
There may be possible a bidirectional relationship between periodontal illness and frailty. Comorbid systemic illnesses, poor bodily functioning, and restricted capacity to self-care in frail older individuals have been implicated as underlying the affiliation between frailty and periodontal illness. As well as, each frailty and periodontal illness even have sturdy associations with inflammatory dysregulation and different age-related pathophysiologic modifications which will equally underlie their improvement and development.
Casein, for Molecular Biology |
|||
MB279-500G | EWC Diagnostics | 1 unit | EUR 387.65 |
Description: Casein, for Molecular Biology |
Tetrahydrofuran |
|||
33112-75 | NACALAI TESQUE | 500ML | EUR 11.69 |
Tetrahydrofuran |
|||
33113-36 | NACALAI TESQUE | 100ML | EUR 9.1 |
Tetrahydrofuran |
|||
33113-65 | NACALAI TESQUE | 500ML | EUR 13.09 |
Tetrahydrofuran |
|||
33116-35 | NACALAI TESQUE | 500ML | EUR 29.05 |
Tetrahydrofuran |
|||
33125-31 | NACALAI TESQUE | 1L | EUR 44.1 |
Tetrahydrofuran |
|||
33139-45 | NACALAI TESQUE | 500ML | EUR 63 |
Tetrahydrofuran |
|||
GK1522-1 | Glentham Life Sciences | 1 | EUR 39.4 |
Tetrahydrofuran |
|||
GK1522-1L | Glentham Life Sciences | 1 l | EUR 84 |
Tetrahydrofuran |
|||
GK1522-2500 | Glentham Life Sciences | 2500 | EUR 71.1 |
Tetrahydrofuran |
|||
GK1522-2500ML | Glentham Life Sciences | 2500 ml | EUR 122.4 |
Tetrahydrofuran |
|||
GK1522-500 | Glentham Life Sciences | 500 | EUR 23.8 |
Tetrahydrofuran |
|||
T293518 | Toronto Research Chemicals | 1l | EUR 57 |
Description: 109-99-9 |
Urea, suitable for molecular biology |
|||
GE1210-1 | Glentham Life Sciences | 1 | EUR 58 |
Urea, suitable for molecular biology |
|||
GE1210-1KG | Glentham Life Sciences | 1 kg | EUR 106.8 |
Urea, suitable for molecular biology |
|||
GE1210-500 | Glentham Life Sciences | 500 | EUR 33.1 |
Urea, suitable for molecular biology |
|||
GE1210-500G | Glentham Life Sciences | 500 g | EUR 76.8 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
|||
CE202 | GeneOn | 25 mg | EUR 70.8 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
|||
CE203 | GeneOn | 100 mg | EUR 126 |
NADPH - Tetrasodium salt (Molecular Biology Grade) |
|||
CE204 | GeneOn | 500 mg | EUR 374.4 |
Tetrahydrofuran 99% |
|||
T04810 | Pfaltz & Bauer | 1L | EUR 238.6 |
Tetrahydrofuran - 1ML |
|||
S-3460 | Scientific Laboratory Supplies | 1ML | EUR 51.3 |
Tetrahydrofuran, 99.9%, for analysis, unstabilised |
|||
GK0126-1 | Glentham Life Sciences | 1 | EUR 83.1 |
Tetrahydrofuran, 99.9%, for analysis, unstabilised |
|||
GK0126-1L | Glentham Life Sciences | 1 l | EUR 136.8 |
Tetrahydrofuran, 99.9%, for analysis, unstabilised |
|||
GK0126-2500 | Glentham Life Sciences | 2500 | EUR 174 |
Tetrahydrofuran, 99.9%, for analysis, unstabilised |
|||
GK0126-2500ML | Glentham Life Sciences | 2500 ml | EUR 246 |
Tetrahydrofuran-d8 |
|||
T293512 | Toronto Research Chemicals | 1g | EUR 230 |
Description: 1693-74-9 |
Tetrahydrofuran-2,2,5,5-d4 |
|||
T293519 | Toronto Research Chemicals | 10mg | EUR 64 |
Description: 20665-63-8 |
2-Mercaptoethanol ?For Molecular Biology |
|||
MB041-100ML | EWC Diagnostics | 1 unit | EUR 9.02 |
Description: 2-Mercaptoethanol ?For Molecular Biology |
2-Mercaptoethanol ?For Molecular Biology |
|||
MB041-500ML | EWC Diagnostics | 1 unit | EUR 26.47 |
Description: 2-Mercaptoethanol ?For Molecular Biology |
Tetrahydrofuran-d8 - 1ML |
|||
S-3461 | Scientific Laboratory Supplies | 1ML | EUR 94.5 |
Tetrahydrofuran 〈H2O<50ppm〉 |
|||
04113-81 | NACALAI TESQUE | 1L | EUR 64.4 |
Tetrahydrofuran 〈H2O<50ppm〉 |
|||
04113-94 | NACALAI TESQUE | 100ML | EUR 14.7 |
2-Methyl Tetrahydrofuran |
|||
24707-100 | Polysciences Europe GmbH | 100g | EUR 55 |
Description: 96-47-9 |
Sodium chloride, suitable for molecular biology |
|||
GE0307-1 | Glentham Life Sciences | 1 | EUR 45.2 |
Sucrose, GlenBiol, suitable for molecular biology |
|||
GC3201-1KG | Glentham Life Sciences | 1 kg | EUR 90 |
Tetrahydrofuran, GlenUltra™, analytical grade, for LC |
|||
GS2560-1 | Glentham Life Sciences | 1 | EUR 176.2 |
Tetrahydrofuran, GlenUltra™, analytical grade, for LC |
|||
GS2560-2500 | Glentham Life Sciences | 2500 | EUR 294 |
Molecular Biology Grade Water for RT-PCR |
|||
ML065-1.5ML | EWC Diagnostics | 1 unit | EUR 7.82 |
Description: Molecular Biology Grade Water for RT-PCR |
VOC Tetrahydrofuran Neat - 10MG |
|||
REVOC176N | Scientific Laboratory Supplies | 10MG | EUR 52.73 |
3-(Chloromethyl)tetrahydrofuran |
|||
B423875 | Toronto Research Chemicals | 500mg | EUR 800 |
Description: 89181-22-6 |
Pyridine, GlenBiol™, suitable for molecular biology with molecular sieve |
|||
GS8780-2500 | Glentham Life Sciences | 2500 | EUR 249.8 |
DTT (Molecular Biology Grade) |
|||
CE131 | GeneOn | 5 g | EUR 93.6 |
DTT (Molecular Biology Grade) |
|||
CE132 | GeneOn | 10 g | EUR 133.2 |
DTT (Molecular Biology Grade) |
|||
CE133 | GeneOn | 25 g | EUR 243.6 |
NAD (Molecular Biology Grade) |
|||
CE196 | GeneOn | 1 g | EUR 72 |
NAD (Molecular Biology Grade) |
|||
CE197 | GeneOn | 5 g | EUR 165.6 |
NBT (Molecular Biology Grade) |
|||
CE209 | GeneOn | 1 g | EUR 123.6 |
NBT (Molecular Biology Grade) |
|||
CE210 | GeneOn | 5 g | EUR 360 |
Sucrose, GlenBiol™, suitable for molecular biology |
|||
GC3201-1 | Glentham Life Sciences | 1 | EUR 45.1 |
Artemether Tetrahydrofuran Acetate |
|||
A777415 | Toronto Research Chemicals | 250mg | EUR 244 |
Description: 181528-64-3 |
EGTA, Molecular Biology Grade |
|||
40500028-2 | Glycomatrix | 50 g | EUR 106.43 |
EGTA, Molecular Biology Grade |
|||
40500028-3 | Glycomatrix | 100 g | EUR 177.58 |
EGTA, Molecular Biology Grade |
|||
40500028-4 | Glycomatrix | 500 g | EUR 603.19 |
EGTA, Molecular Biology Grade |
|||
40500028-5 | Glycomatrix | 1 kg | EUR 912.98 |
EGTA, Molecular Biology Grade |
|||
40500028-6 | Glycomatrix | 2 kg | EUR 1687.94 |
DMSO, Molecular Biology Grade |
|||
40470006-1 | Glycomatrix | 100 mL | EUR 88.18 |
DMSO, Molecular Biology Grade |
|||
40470006-2 | Glycomatrix | 250 mL | EUR 150.19 |
DMSO, Molecular Biology Grade |
|||
40470006-3 | Glycomatrix | 500 mL | EUR 279.26 |
BCIP (Molecular Biology Grade) |
|||
CE108 | GeneOn | 250 mg | EUR 75.6 |
BCIP (Molecular Biology Grade) |
|||
CE109 | GeneOn | 1 g | EUR 108 |
DAPI (Molecular Biology Grade) |
|||
CE117 | GeneOn | 5 mg | EUR 72 |
DAPI (Molecular Biology Grade) |
|||
CE118 | GeneOn | 25 mg | EUR 159.6 |
DAPI (Molecular Biology Grade) |
|||
CE119 | GeneOn | 100 mg | EUR 382.8 |
Tris (Molecular Biology Grade) |
|||
CE237 | GeneOn | 500 g | EUR 106.8 |
Tris (Molecular Biology Grade) |
|||
CE238 | GeneOn | 1 kg | EUR 153.6 |
Tris (Molecular Biology Grade) |
|||
CE239 | GeneOn | 5 kg | EUR 535.2 |
Tetrahydrofuran-2,5-dicarboxylic Acid |
|||
T293525 | Toronto Research Chemicals | 1g | EUR 167 |
Pyridine, GlenBiol™, suitable for molecular biology |
|||
GS6659-2500 | Glentham Life Sciences | 2500 | EUR 240.3 |
Pyridine, GlenBiol™, suitable for molecular biology |
|||
GS6659-500 | Glentham Life Sciences | 500 | EUR 95.8 |
Formamide, GlenBiol™, suitable for molecular biology |
|||
GS9663-100 | Glentham Life Sciences | 100 | EUR 48.9 |
2-Aminomethyl tetrahydrofuran 97% |
|||
A24940 | Pfaltz & Bauer | 25G | EUR 129.43 |
CHAPS (Molecular Biology Grade) |
|||
CE114 | GeneOn | 1 g | EUR 66 |
CHAPS (Molecular Biology Grade) |
|||
CE115 | GeneOn | 5 g | EUR 157.2 |
CHAPS (Molecular Biology Grade) |
|||
CE116 | GeneOn | 25 g | EUR 492 |
HEPES (Molecular Biology Grade) |
|||
CE171 | GeneOn | 100 g | EUR 98.4 |
HEPES (Molecular Biology Grade) |
|||
CE172 | GeneOn | 500 g | EUR 268.8 |
HEPES (Molecular Biology Grade) |
|||
CE173 | GeneOn | 1 kg | EUR 424.8 |
Water (Molecular Biology Grade) |
|||
CE243 | GeneOn | 500 ml | EUR 62.4 |
Water (Molecular Biology Grade) |
|||
CE244 | GeneOn | 1 l | EUR 67.2 |
Molecular Biology Grade Water |
|||
ML024-100ML | EWC Diagnostics | 1 unit | EUR 3.54 |
Description: Molecular Biology Grade Water |
Molecular Biology Grade Water |
|||
ML024-10X100ML | EWC Diagnostics | 1 unit | EUR 29.52 |
Description: Molecular Biology Grade Water |
Molecular Biology Grade Water |
|||
ML024-10X500ML | EWC Diagnostics | 1 unit | EUR 87.91 |
Description: Molecular Biology Grade Water |
Molecular Biology Grade Water |
|||
ML024-500ML | EWC Diagnostics | 1 unit | EUR 12.56 |
Description: Molecular Biology Grade Water |
Molecular Biology Grade Water |
|||
ML064-100ML | EWC Diagnostics | 1 unit | EUR 3.67 |
Description: Molecular Biology Grade Water |
Molecular Biology Grade Water |
|||
ML064-10X100ML | EWC Diagnostics | 1 unit | EUR 25.38 |
Description: Molecular Biology Grade Water |
Molecular Biology Grade Water |
|||
ML064-500ML | EWC Diagnostics | 1 unit | EUR 10.81 |
Description: Molecular Biology Grade Water |
VOC Std Tetrahydrofuran 2000ug/mL in MeOH - 1ML |
|||
REVOC176 | Scientific Laboratory Supplies | 1ML | EUR 74.91 |
2,5-Bishydroxymethyl Tetrahydrofuran |
|||
B446375 | Toronto Research Chemicals | 100mg | EUR 170 |
Description: 104-80-3 |
2,5-Bis(aminomethyl)tetrahydrofuran |
|||
B404300 | Toronto Research Chemicals | 100mg | EUR 253 |
Description: 66918-21-6 |
Agarose, Molecular Biology Grade |
|||
40100164-1 | Bio-WORLD | 25 g | Ask for price |
Agarose, Molecular Biology Grade |
|||
40100164-2 | Bio-WORLD | 50 g | Ask for price |
Agarose, Molecular Biology Grade |
|||
40100164-3 | Bio-WORLD | 100 g | Ask for price |
Agarose, Molecular Biology Grade |
|||
40100164-4 | Bio-WORLD | 500 g | Ask for price |
Agarose, Molecular Biology Grade |
|||
40100164-5 | Bio-WORLD | 1 kg | Ask for price |
Glycine (Molecular Biology Grade) |
|||
CE158 | GeneOn | 1 kg | EUR 84 |
Glycine (Molecular Biology Grade) |
|||
CE159 | GeneOn | 5 kg | EUR 228 |
Tween20 (Molecular Biology Grade) |
|||
CE242 | GeneOn | 1 l | EUR 106.8 |
Agarose (Molecular Biology Grade) |
|||
abx299715-100g | Abbexa | 100 µg | Ask for price |
Agarose (Molecular Biology Grade) |
|||
abx299715-20g | Abbexa | 20 µg | EUR 525 |
Agarose (Molecular Biology Grade) |
|||
abx299715-50g | Abbexa | 50 µg | Ask for price |
2-(Tetrahydrofuran-3-yl)acetaldehyde |
|||
T293605 | Toronto Research Chemicals | 2.5g | EUR 1200 |
Description: 1072-94-2 |
Tetrahydrofuran, GlenDry™, anhydrous |
|||
GS6029-1 | Glentham Life Sciences | 1 | EUR 121 |
Tetrahydrofuran, GlenDry™, anhydrous |
|||
GS6029-100 | Glentham Life Sciences | 100 | EUR 66.4 |
Tetrahydrofuran, GlenDry™, anhydrous |
|||
GS6029-2500 | Glentham Life Sciences | 2500 | EUR 223 |
Tetrahydrofuran, GlenDry™, anhydrous |
|||
GS6029-500 | Glentham Life Sciences | 500 | EUR 88.7 |
2-(4-Hydroxybutoxy)tetrahydrofuran |
|||
H819500 | Toronto Research Chemicals | 100mg | EUR 295 |
Description: 64001-06-5 |
Lysozyme (Molecular Biology Grade) |
|||
CE188 | GeneOn | 1 g | EUR 70.8 |
Lysozyme (Molecular Biology Grade) |
|||
CE189 | GeneOn | 10 g | EUR 247.2 |
Lysozyme (Molecular Biology Grade) |
|||
CE189L | GeneOn | 50 g | EUR 310 |
Lysozyme (Molecular Biology Grade) |
|||
CE189XL | GeneOn | 250 g | EUR 1050 |
Tetrahydrofuran-2,5-dicarboxylic Acid (>80%) |
|||
T222765 | Toronto Research Chemicals | 1g | EUR 81 |
Description: 6338-43-8 |
Tetrahydrofuran Fentanyl Hydrochloride |
|||
T293476 | Toronto Research Chemicals | 10mg | EUR 1455 |
OORA00229-1L - Molecular Biology Grade UltraPure Water |
|||
OORA00229-1L | Aviva Systems Biology | 1L | EUR 149 |
OORA00230-1L - Molecular Biology Grade UltraPure Water |
|||
OORA00230-1L | Aviva Systems Biology | 1L | EUR 279 |
2-((Tetrahydrofuran-2-yl)methoxy)phenol |
|||
T689455 | Toronto Research Chemicals | 10mg | EUR 201 |
Description: 41516-19-2 |
Dimethylformamide, GlenBiol™, suitable for molecular biology with molecular sieve |
|||
GS3406-2500 | Glentham Life Sciences | 2500 | EUR 116.2 |
"10X PBS, Molecular Biology Grade" |
|||
ML023-100ML | EWC Diagnostics | 1 unit | EUR 17.65 |
Description: "10X PBS, Molecular Biology Grade" |
10X PBS, Molecular Biology Grade |
|||
ML023-500ML | EWC Diagnostics | 1 unit | EUR 38.97 |
Description: 10X PBS, Molecular Biology Grade |
"10X TBS, Molecular Biology Grade" |
|||
ML029-100ML | EWC Diagnostics | 1 unit | EUR 19.04 |
Description: "10X TBS, Molecular Biology Grade" |
"10X TBS, Molecular Biology Grade" |
|||
ML029-500ML | EWC Diagnostics | 1 unit | EUR 40.48 |
Description: "10X TBS, Molecular Biology Grade" |
10X TBS, Molecular Biology Grade |
|||
ML029-6X500ML | EWC Diagnostics | 1 unit | EUR 138.56 |
Description: 10X TBS, Molecular Biology Grade |
Cobalt(II) chloride-1,5-Tetrahydrofuran |
|||
GX7281-10 | Glentham Life Sciences | 10 | EUR 88.9 |
Cobalt(II) chloride-1,5-Tetrahydrofuran |
|||
GX7281-25 | Glentham Life Sciences | 25 | EUR 131.1 |
Agarose, low EEO, GlenBiol, suitable for molecular biology |
|||
GE6258-100G | Glentham Life Sciences | 100 g | EUR 217.2 |
×
Investigating age-related modifications in immune cells that regulate irritation could result in a greater understanding of age-related illness and will result in therapeutic targets for the improved administration of frailty and periodontal illness.
Tags: 3x flag peptide 3xflag sequence avi tag sequence avitag bl21 codon plus cherry plasmid dab substrate dh10b dh5 alpha dna sequencing software electrocompetent flag sequence flag tag dna sequence flag tag sequence gel loading buffer gfp vector gibson assembly master mix gibson assembly primer design gibson cloning kit jm109 loading buffer maxiprep miniprep miniprep kit order cdna order pcr primers p24 elisa paav plasmid pbabepuro pcdna3 pcdna3 1 hygro pcdna3 2 pcdna3 invitrogen pcdna3.1 vector map pcdna4 pcmv vector pet duet vector pet plasmids pet26b peyfpc1 pgex 2t pgk puro pgkpuro plasmid midi kit plenti gfp plenti plasmid plenti vector pmcherry sequence pshuttle cmv puro sequence pyes vector rosetta competent cells sumo tag tbe buffer tdtomato turbo gfp antibody turborfp sequence